POCT CTnI Cardiac Marker Test Kit 15 Minutes For IFP 2000 Analyzer

15 Minutes POCT CTnI Cardiac Marker Test Kit For IFP-2000 Analyzer

Assay procedure

Please read the applicable instrument manual and this manual carefully before use. The reagent should be returned to room temperature before testing, and the testing process should also be performed at room temperature.

1.Inspection steps

1.1 For models of IFP-2000 dry fluorescence immunoassay analyzers produced by Xincheng Bio and models of AFS-1000 and AFS2000A dry fluorescent immunoassay analyzers produced by Labsim, the operation steps are as follows:

1. 1.1 Open the kit, check that the batch number of the test card, the batch number of the buffer solution and the id chip are consistent, and insert the id chip into the instrument chip port.

1.1.2 Take 75μL serum / plasma / whole blood to add to the centrifuge tube containing buffer, sufficiently mixed for 1 minute.

Note that the mixing time should be accurate, otherwise the results may be inaccurate.

1.1.3 Take 75μL of the above sample solution and add it to the sample hole of the test card.

1.1.4 Select the appropriate detection mode for detection

Quick mode: After adding samples to the test card and manually timing for 15 minutes, insert the test card into the instrument immediately, and the instrument will immediately detect and display the result.

Note that the timing should be accurate, otherwise the result may be inaccurate.

Standard mode: Click the "Test" button, and insert the test card into the instrument within 1 minute of the countdown, the instrument will continue the countdown, and automatically detect and display the result at 15 minutes.

1.1.5 After the test is completed, the test card should be taken out of the instrument and handled according to regulations.

1.2 Operation steps of Auto-HFIAS 1000, HFD-Station dry fluorescence immunoassay analyzers are as follow:

1.2.1 Open the kit and insert the test card into the entrance and exit of the analyzer.

1.2.2 Take 150μL ~ 250μL serum/plasma/whole blood into the sample hole of the test card .

1. 2.3 Select the appropriate sample detection mode for detection in the instrument operation interface.

1. 2.4 After the test is completed, the test card should be taken out of the instrument and handled according to regulations.

2. Calculation

Use multi-point non-linear/semi-logarithmic calibration mode, with spline function as the calculation mode, and draw the dose/response curve based on the value of the calibrator and the absorbance change. The concentration of cTnI in the sample can be based on the absorbance change in the dose/response calculated on the curve.

Applicable instrument

The models produced by Sichuan Xincheng Biological Co., LTD:

IFP-2000 dry fluorescence immunoassay analyzer,