This reagent forms a double antibody sandwich complex with cTnI, and is detected by a
fluorescence detector, so as to realize the quantitative determination of cTnI concentration in
serum, plasma and whole blood. After the sample to be tested is evenly mixed with the buffer, it
is dripped into the sample hole of the test card. The sample reacts with the fluorescently labeled
antigen-antibody complex, which is then chromatographed to nitrocellulose on the plain film, it
reacts specifically with the coated mouse anti-human cTnI monoclonal antibody and is captured.
The amount of captured fluorescent complex is positively correlated with the concentration of
cTnI in the sample. The fluorescence intensity is detected and converted to the concentration of
cTnI according to the calibration curve calibrated on the ID chip or test card.
The kit consists of a test card, buffer, and ID chip (optional). among them:
Test card: Consists of a reagent strip and a plastic box. The main components on the reagent
strip are: nitrocellulose membrane, absorbent paper, PVC board and marking pad; the reagent
strip is coated with mouse anti-human cTnI monoclonal antibody (each reagent strip contains 0.11μg～1.12μg) and Rabbit IgG (0.20μg～1.50μg on each reagent strip), fluorescently labeled
mouse anti-human cTnI monoclonal antibody (0.04μg～0.24μg on each reagent strip),
fluorescently labeled rabbit IgG (each reagent strip The bar contains 0.20μg～1.50μg).
Buffer: Tris buffer (Tris) (50mmol/L).
ID chip: Contains basic project information, batch information and calibration curve.
Applicable to IFP-2000, IFP-3000 produced by Xincheng Bio and AFS-1000, AFS2000A models
produced by Labsim. It is equipped with ID chip ; the ID chip stores basic project information,
batch information and calibration curve, and the barcode on the test card only contains batch
information. Applicable to Xincheng Bio 's Auto-HFIAS 1000 and HFD-Station models without ID
chip . The basic project information, batch information and calibration curve are marked on the
test card in the form of a QR code.
The measurement system can be traced to the cTnI measurement system approved by NMPA.
The components in the kits with different batch numbers cannot be interchanged or mixed.
Storage and stability
The test card and buffer can be stored at 2℃～30℃ for 24 months, and the buffer solution can
be stored at 2℃～30℃ for 2h after opening the bottle . The aluminum foil bag of the test
card should be used within 2 hours after it is opened. Transportation and storage should be
carried out at 2℃～30℃.
The models produced by Sichuan Xincheng Biological Co., LTD:
IFP-2000, IFP-3000,Auto- HFIAS 1000, HFD-Station dry fluorescence immunoassay analyzer,
The models produced by Guangzhou Labsim Biotechnology Co., Ltd.:
AFS-1000, AFS2000A dry fluorescence immunoassay analyzer.